Master of Science
Objectives: To determine changes in prostate microparticle (PMP) concentrations in men with prostate cancer (PCa) after digital rectal examination (DRE), after radical prostatectomy (RP), and at follow-up.
Materials and Methods: 22 men were recruited before RP. Four blood specimens were collected – baseline (specimen 1), post-DRE (specimen 2), immediately post-RP (specimen 3), and follow-up (specimen 4). Pre- and post-DRE urine was collected (Specimen A and B respectively). Flow cytometric analysis of biofluids was performed with fluorescent-labeled antibodies against prostate-specific membrane antigen (PSMA) and polysialic acid. Total MP (TMP) and dual positive (PMP) events per µl of plasma or urine were recorded.
Results: Median TMPs from specimen 1 to 4 were 3 005 500, 1 600 600, 976 353, and 3 951 400 events/µl, respectively (p
Conclusions: Proportional numbers of PMPs in men with PCa are significantly increased following DRE and RP.
Dewar, Malcolm James, "Prostate Cancer Microparticles in Men Undergoing Radical Prostatectomy" (2017). Electronic Thesis and Dissertation Repository. 5140.
Available for download on Wednesday, January 01, 2020