Mitochondria-Targeted Hydrogen Sulphide Donors Protect Renal Cells From Hypoxia Re-Oxygenation Injury
Master of Science
Dr. Alp Sener
Introduction: Hypoxia re-oxygenation in kidney transplantation affects the outcome. Hydrogen sulphide (H2S), (the newest Gasotransmitter), showed significant protective effect on renal transplantation induced IRI. Our objective was to determine if new mitochondria targeted H2S donor molecule (AP39) would be more efficacious in protecting renal cells against IRI compared to the non-specific H2S donor molecule GYY4137. We hypothesized that AP39 would be more potent.
Methods: in vitro porcine kidney tubular epithelial cells (LCC-PK1) were exposed to warm hypoxia, without treatment (Control), with AP39 or GYY4137 followed by re-oxygenation.
Results: 200nM of AP39 protected the cells and maintained a high viability. AP39 was superior to GYY4137. Significant reduction of Apoptosis and ROS were noted in AP39 samples when compared to control. Both BCL2 and BID genes did not show any significant changes, compared to the control and GYY4137 samples.
Conclusion: AP39 is protective and superior to GYY4137 in renal IRI.
Aboalsamh, Ghaleb A., "Mitochondria-Targeted Hydrogen Sulphide Donors Protect Renal Cells From Hypoxia Re-Oxygenation Injury" (2015). Electronic Thesis and Dissertation Repository. 2912.