Date of Award


Degree Type


Degree Name

Master of Science




Dr. Brent Sinclair

Second Advisor

Dr. Marc-André Lachance

Third Advisor

Dr. Mark Bernards


In order to understand potential for northward spread of the emerald ash borer in North America, the cold tolerance strategy and mechanisms involved in the cold tolerance of the emerald ash borer were investigated, and seasonal changes in these mechanisms monitored. Seasonal changes in energy stores were also measured. Emerald ash borers survive winter as freeze-intolerant prepupae. In winter, they have low supercooling points (~ 30 °C), which are achieved by accumulating high concentrations of glycerol (~4 M) in their body fluids and by the synthesis of antifreeze agents. Cuticular waxes help prevent inoculation from external ice. Triglyceride stores are depleted rapidly in spring, and glycogen increases slightly. Increased developmental time in December suggests the presence of a prepupal diapause. Overall, the emerald ash borer is well-equipped to survive cold and northern ash populations are likely under threat.



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