Date of Award


Degree Type


Degree Name

Master of Science


Microbiology and Immunology


Dr. Peta O'Connell

Second Advisor

Dr. Gregory Dekaban


Magnetic resonance imaging (MRI) can be used to track DC in vivo because it non-invasively combines critical anatomical detail and sensitivity. To identify cells using MRI it is necessary tolabel them with a contrast agent. DC were labelled with superparamagnetic iron oxide (SPIO) ∞ntrast agents for in vivo imaging. It was found that Feridex was taken up intracellularly and has minimal impact on the DC size and expression of cell maturation markers. Following injection into mice, the labelledDC could be detected by in vivo MRI in draining lymph nodes. The MRI data was consistent with histological analysis which confirmed the presence of Feridex-labelled cells. These results suggest that (1 ) DC can be labelled with Feridex with limited affect, (2) Feridex- labelled DC can be imaged in vivo with MRI, and (3) the distribution of Feridex- labelled cells in lymph nodes is consistent with MRI.



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