Richard Miron

Date of Award


Degree Type


Degree Name

Master of Science


Anatomy and Cell Biology


Dr. Hamilton

Second Advisor

Dr. Jeff Dixon


Modifications of implant surface topography and chemistry have proven a means to enhance osseointegration, a process that ensures the stability of bone-contacting devices including titanium dental implants. Enamel matrix derivative (EMD) extracted from developing porcine teeth, has been shown to promote regeneration of bone. The aim of the present study was to evaluate the effect of EMD on the attachment, proliferation and differentiation of osteoblasts on titanium surfaces in vitro. Pickled (smooth) and SLA (Sand-blasted with Large grit followed by Acid etching) titanium discs were coated with EMD or left uncoated. Primary rat calvarial osteoblasts were cultured on each surface 1 hour to 4 weeks. EMD significantly increased cell spreading and proliferation at time points ranging from 3 to 7 days on both topographies. Alkaline phosphatase activity was significantly increased on EMD-coated titanium compared with titanium alone. Moreover, there was a 4-fold increase in levels of mRNA encoding bone sialoprotein and osteocalcin in osteoblasts cultured on EMD-coated titanium surfaces compared with uncoated surfaces. We conclude that coating of titanium with EMD enhances the proliferation and differentiation of osteoblasts irrespective of the titanium substratum topography. Therefore, EMD may increase the speed and quality of osseointegration

around bone contacting implants in vivo.



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