Date of Award
2009
Degree Type
Thesis
Degree Name
Master of Engineering Science
Program
Biomedical Engineering
Supervisor
Dr. Wankei Wan
Second Advisor
Dr. Derek Boughner
Abstract
Tissue engineering is recognized as a promising alternative to conventional reconstructive materials and donor tissues, which are in short supply. The effective use of nanofibers as tissue scaffolds relies on the localized delivery of signaling proteins, providing biochemical cues for tissue regeneration. This study aimed to fabricate bioactive scaffolds by incorporating proteins for controlled release through a coaxial electrospinning process. It was used to fabricate Poly (s-Caprolactone) fibers within which bioactive proteins were encapsulated. Scaffolds were characterized using SEM and confocal microscopy to verify uniformity and continuity of protein encapsulation. Protein release kinetics were evaluated using fluorescence spectroscopy and a Bradford protein assay. Cell culture studies using radial arterial cells were performed on scaffolds containing either TGF-pl or PDGF-BB. The protein release study suggested diffusive transport was sustained over a 72-hour period and significantly improved cell proliferation. The present results provide a basis for further optimization of scaffold
processing parameters.
Recommended Citation
Haley, Jason P., "BIOACTIVE SCAFFOLDS FOR TISSUE ENGINEERING" (2009). Digitized Theses. 3811.
https://ir.lib.uwo.ca/digitizedtheses/3811