Date of Award


Degree Type


Degree Name

Master of Science


Microbiology and Immunology


Dr. David E. Heinrichs


Staphylococcus aureus harbours several iron acquisition systems, allowing for survival within the iron-restricted host environment. Five such systems have been identified in S. aureus; it is unknown why S. aureus maintains so many of these systems. Studies have shown that some iron transport proteins might be differentially expressed in different organs in vivo. The purpose of this study was to construct an in vivo expression technology (IVET) system to identify temporal expression patterns of S. aureus iron transport systems. An IVET utilizing the CrelloxP recombination system was constructed for use in S. aureus. Using genome-integrated and multi-copy ere constructs driven by the S. aureus fhuCBG promoter, resolution of an in vitro reporter cassette was determined. Although fhuCBG driven ere expression was confirmed using the multi-copy ere constructs, no detectable Cre was expressed by genome-integrated cre constructs.



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