Date of Award


Degree Type


Degree Name

Master of Science




Dr. Sashko Damjanovski


Development in the frog, Xenopus laevis, requires the utilization of yolk glyco- lipo-proteins in a temporally- and spatially-dependent manner. The metabolism of the yolk produces H20 2, a potent Reactive Oxygen Species (ROS). Peroxiredoxins (Prdxs) are a family of 6 anti-oxidant enzymes that, amongst other roles, reduce H20 2. Prdxs reduce H20 2through a thiol-redox reaction at conserved cysteine residues, which results in the creation of disulfide bonds. Recently the thiol-redox reaction of Prdxs has also been implicated in several cell signaling systems. Herein is a report of the cloning and expression patterns during development of 6 peroxiredoxin homologs from the frog X. laevis. Sequence analysis has confirmed their identity as well as their evolutionary relationship with peroxiredoxins from several other species. Using RT-PCR and in situ hybridization analysis we have shown that there is early, robust and unique expression patterns for all six homologs during development. All six X. laevis peroxiredoxins are expressed in neural regions including the brain, and eyes, as well as the somites. Different expression patterns for each peroxiredoxin are also observed in the pronephric region, including the proximal and distal tubules and the Wolffian duct. Expression of several peroxiredoxins was also observed in the blood precursors and the olfactory placode. These results suggest important roles for all six peroxiredoxins during early development. These roles may be restricted to their functions as anti-oxidant enzymes, but may also be related to their emerging roles in redox signaling, which is discussed herein.



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