Analysis of chondrogenesis using micromass cultures of limb mesenchyme

Authors

T. Michael Underhill, The University of British Columbia
Helen J. Dranse, The University of British Columbia
Lisa M. Hoffman, The University of British Columbia

Document Type

Article

Publication Date

1-1-2014

Journal

Methods in Molecular Biology

Volume

1130

First Page

251

Last Page

265

URL with Digital Object Identifier

10.1007/978-1-62703-989-5_19

Abstract

High-density micromass cultures of embryonic mesenchymal cells have proved to be an invaluable model for studying the entire chondrogenic program, from precartilaginous condensations through to chondrocyte hypertrophy. This culture model also provides a powerful system in which to explore the function of various factors in the commitment and differentiation of mesenchymal cells to the chondrogenic lineage. In this regard, micromass cultures provide a consistent and robust model for investigating the effects of genetic manipulations on skeletal phenotypes and for delineating their molecular basis. In this methods chapter, the derivation and use of micromass cultures from murine limb buds are described, but these techniques are also applicable to other organisms and mesenchymal cell sources. © 2014 Springer Science+Business Media, LLC.