Bone and Joint Institute

Title

Decellularized adipose tissue scaffolds for soft tissue regeneration and adipose-derived stem/stromal cell delivery

Document Type

Article

Publication Date

1-1-2018

Journal

Methods in Molecular Biology

Volume

1773

First Page

53

Last Page

71

URL with Digital Object Identifier

10.1007/978-1-4939-7799-4_6

Abstract

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Surgically discarded adipose tissue is not only an abundant source of multipotent adipose-derived stem/stromal cells (ASCs) but can also be decellularized to obtain a biomimetic microenvironment for tissue engineering applications. The decellularization methods involve processing excised fat through a series of chemical, mechanical, and enzymatic treatment stages designed to extract cells, cellular components, and lipid from the tissues. This process yields a complex 3D bioscaffold enriched in collagens that mimics the biochemical and biomechanical properties of the native extracellular matrix (ECM). For ASC culture and delivery, decellularized adipose tissue (DAT) provides a cell-supportive platform that is conducive to adipogenesis. While DAT can be applied in its intact form as an off-the-shelf adipogenic matrix, it can also be used as an ECM source for the fabrication of an array of other scaffold formats including adipose ECM-derived microcarriers and porous foams. In this chapter, we describe the methods developed in our lab to decellularize human adipose tissue and to further process it into a variety of scaffolding materials for a range of applications in soft tissue regeneration, wound healing, and cell culture.

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