Multimodal measurements of brain tissue metabolism and perfusion in a neonatal model of hypoxic-ischaemic injury
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
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© 2019 SPIE. This is the first multimodal study of cerebral tissue metabolism and perfusion post-hypoxic-ischaemic (HI) brain injury with broadband near-infrared spectroscopy (bNIRS), diffuse correlation spectroscopy (DCS), positron emission tomography (PET) and magnetic resonance spectroscopy (MRS). In 5 piglet models of HI, we measured cerebral tissue saturation (StO2), cerebral blood flow (CBF), cerebral oxygen metabolism (CMRO2), changes in the mitochondrial oxidation state of cytochrome-c-oxidase (oxCCO), cerebral glucose metabolism (CMRglc), and tissue biochemistry (Lac+Thr/tNAA). At baseline, the parameters measured were: 64±6 % StO2, 35±11 ml/100g/min CBF, and 2.0±0.4 μmol/100g/min CMRO2. After HI the parameters measured were: 68±6% StO2, 35±6 ml/100g/min CBF, 1.3±0.1 μmol/100g/min CMRO2, 0.4±0.2 Lac+Thr/tNAA, and 9.5±2.0 CMRglc. This study demonstrates the capacity of a multimodal set up to interrogate the pathophysiology of HIE using a combination of optical methods, MRS, and PET.