Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Medical Biophysics

Supervisor

Dr. David Sanders

2nd Supervisor

Dr. Gediminas Cepinskas

Joint Supervisor

Abstract

Complex regional pain syndrome type-I (CRPS-I) is a debilitating pain disorder often occurring secondary to distal extremity trauma. Its pathophysiology is not well understood; however, microvascular dysfunction is proposed as an important factor in its development and maintenance. Using a rodent model, we tested an automated gait analysis system (CatWalk™) to examine functional changes. In addition, the use of carbon monoxide releasing molecule-3 (CORM-3), a compound known to be a potent vasodilator and anti-inflammatory agent, was also tested as a treatment of CRPS-I-like symptoms. Using the CatWalk™ system, we observed significant changes in gait parameters post-injury, several of which persisted throughout the 14-day experiment. CORM-3 administration significantly reduced mechanical allodynia symptoms, as demonstrated through the restoration of withdrawal thresholds during mechanical stimulation testing. Functional deficits were not restored after CORM-3 application; however, trends for improvement were observed. CORM-3 has relevance as a potential therapy to alleviate symptoms associated with CRPS-I.

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