Title

De Novo Interpretation of Tandem Mass Spectra

Document Type

Article

Publication Date

3-2009

Journal

Curr Protoc Bioinformatics

Volume

Chapter 13

URL with Digital Object Identifier

10.1002/0471250953.bi1310s25

Abstract

De novo sequencing is an effective method for identifying unknown peptide sequences from their tandem mass spectra. This unit briefly introduces how this can be done manually. A protocol for using the PEAKS online software for automated de novo sequencing is described. Finally, we show how to use the PEAKS scores to validate the de novo sequencing results.

Notes

Published as part of Chapter 13 (Unit 13.10) of Current Protocols in Bioinformatics.