Title

Enzymatic Site-Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling

Document Type

Article

Publication Date

6-22-2010

Journal

Angewandte Chemie International Edition

Volume

49

Issue

30

First Page

5170

Last Page

5173

URL with Digital Object Identifier

http://dx.doi.org/10.1002/anie.201001240

Abstract

Pass and click: Protein methylation is an important posttranslational modification. Because the methyl group is a poor reporter group, new methods are needed to analyze methyltransferase substrates. A S-adenosyl-L-methionine-based cofactor was synthesized and used for the site-specific functionalization of proteins with alkynes by methyltransferases (first step) and subsequent labeling through CuAAC click chemistry (second step; see scheme).