Author Information

Dong Kyung LeeFollow

Department

biology

Program

master

Year

2018

Supervisor Name

David Smith

Supervisor Email

dsmit242@uwo.ca

Abstract Text

Genomic Analysis of Mitochondria of Metschnikowia

Background

Our current understanding of mitochondrial genomes is biased toward metazoans, which represent the majority of sequenced mitochondrial DNAs (mtDNAs). The high diversity in size, shape, and gene synteny among yeast mitochondrial genomes make them an appealing alternative to those of metazoans for studying genomic diversity and evolution. Recently, more than 60 draft-genome assemblies of large-spored Metschnikowia species have been determined. Complete mitochondrial genomes of these yeasts, however, are yet to be constructed and characterized. Therefore, I propose to construct and analyze the mitochondrial genomes of all 61 strains of large-spored Metschnikowia species.

Hypothesis

The initial hypothesis is that the features of the mitochondrial genomes will follow species relatedness.

Methods

I have found that the sequencing read databases of these species are highly enriched in mitochondrial sequences with a high adenine and thymine content. These properties will be used to help isolate mitochondrial reads and assemble them into intact and complete genome sequences.

Results

The preliminary analyses showed that the mtDNAs from Metschnikowia can be circular or linear, and it will be of interest to find out whether the distribution of circular and linear forms follows phylogenetic lines. Also, genome sizes have shown a four-fold range so far, but it is not yet known if the size distribution will be consistent with the phylogeny. Similarly, patterns of both gene synteny and genome topology varies between early and late emerging species. Furthermore, cox1 and cob contain multiple introns that varies extensively in numbers and can be incredibly high (≈20).

In progress (data not fully collected)

Dietary Restrictions

no cilantro

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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Genomic Analysis of Mitochondria of Metschnikowia

Genomic Analysis of Mitochondria of Metschnikowia

Background

Our current understanding of mitochondrial genomes is biased toward metazoans, which represent the majority of sequenced mitochondrial DNAs (mtDNAs). The high diversity in size, shape, and gene synteny among yeast mitochondrial genomes make them an appealing alternative to those of metazoans for studying genomic diversity and evolution. Recently, more than 60 draft-genome assemblies of large-spored Metschnikowia species have been determined. Complete mitochondrial genomes of these yeasts, however, are yet to be constructed and characterized. Therefore, I propose to construct and analyze the mitochondrial genomes of all 61 strains of large-spored Metschnikowia species.

Hypothesis

The initial hypothesis is that the features of the mitochondrial genomes will follow species relatedness.

Methods

I have found that the sequencing read databases of these species are highly enriched in mitochondrial sequences with a high adenine and thymine content. These properties will be used to help isolate mitochondrial reads and assemble them into intact and complete genome sequences.

Results

The preliminary analyses showed that the mtDNAs from Metschnikowia can be circular or linear, and it will be of interest to find out whether the distribution of circular and linear forms follows phylogenetic lines. Also, genome sizes have shown a four-fold range so far, but it is not yet known if the size distribution will be consistent with the phylogeny. Similarly, patterns of both gene synteny and genome topology varies between early and late emerging species. Furthermore, cox1 and cob contain multiple introns that varies extensively in numbers and can be incredibly high (≈20).