Title of Research Output

Oxidative Stress in Gryllus veletis

Faculty

Science

Supervisor Name

Brent Sinclair

Keywords

Freeze tolerance, insects, oxidative stress, acclimation, low temperature biology

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Description

The cricket Gryllus veletis is an emerging model for freeze-tolerance, being able to withstand internal ice formation. When freezing, ice crystals form extracellularly around cells preventing gases from entering. Upon thawing, oxygen can then flood into the cell, most likely increasing the production of Reactive Oxygen Species (ROS) which can cause oxidative stress. Post-thawing crickets can repair the oxidative stress with the help of different antioxidants. For Zainab and I’s research project, we explored oxidative stress in G. veletis during freezing-thawing and post-thawing. In this short series of videos done in partnership with Zainab, I will briefly go over our experimental design, methods and hypotheses.

Acknowledgements

Thank you to the Western USRI program for your support. A special thank you to the Sinclair Lab for their support and encouragement throughout this internship. Thank you, Dr. Sinclair, Stefane Saruhashi, Kaitlyn Ludba and Lamees Mohammad for your mentorship, advice and support throughout this internship. I could not have done it without any of you!

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Document Type

Video

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Oxidative Stress in Gryllus veletis

The cricket Gryllus veletis is an emerging model for freeze-tolerance, being able to withstand internal ice formation. When freezing, ice crystals form extracellularly around cells preventing gases from entering. Upon thawing, oxygen can then flood into the cell, most likely increasing the production of Reactive Oxygen Species (ROS) which can cause oxidative stress. Post-thawing crickets can repair the oxidative stress with the help of different antioxidants. For Zainab and I’s research project, we explored oxidative stress in G. veletis during freezing-thawing and post-thawing. In this short series of videos done in partnership with Zainab, I will briefly go over our experimental design, methods and hypotheses.