Modulation of adhesion of uropathogenic enterococcus faecalis to human epithelial cells in vitro by lactobacillus species
Microbes and Infection
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Two mammalian antimicrobial peptides, FA-LL-37 and cecropin P1, were tested for activity against six uropathogens and five Lactobacillus strains by broth microdilution assay. Both peptides inhibited Escherichia coli at 25 μM (FA-LL-39), and 1.56 μM (cecropin P1), Pseudomonas aeruginosa (12.5 μM, and 25 μM), and Klebsiella pneumoniae, (50 μM, and 1.56 μM), but not Enterococcus faecalis and Staphylococcus epidermidis. FA-LL-37 acted bacteriocidally against E. coli and bacteriostatically against the other two Gram-negative organisms. Cecropin P1 was bacteriocidal to all susceptible bacteria. Lactobacilli were resistant to both peptides, with the exception of poultry isolate Lactobacillus fermentum B-54, which was susceptible to FA-LL- 37 at 100 μM. The differential activities of these peptides toward Gram- negative uropathogens versus urogenital lactobacilli demonstrate their potential as a topical treatment for urinary tract infections. In addition, production of such peptides in vivo could be a natural mechanism to aid in the maintenance of the lactobacilli-dominated urogenital flora at the expense of pathogens. (C) 2000 Editions scientifiques et medicales Elsevier SAS.