Microbiology & Immunology Publications
Title
Development and pilot evaluation of a novel probiotic mixture for the management of seasonal allergic rhinitis
Document Type
Article
Publication Date
8-1-2008
Journal
International Journal of Hydrogen Energy
Volume
33
Issue
15
First Page
4064
Last Page
4073
URL with Digital Object Identifier
10.1016/j.ijhydene.2008.05.069
Abstract
The effect of heat treatment at different temperatures on two types of inocula, activated sludge and anaerobically digested sludge, was investigated in batch cultures. Heat treatments were conducted at 65, 80 and 95 °C for 30 min. The untreated inocula produced less amount of hydrogen than the pretreated inocula, with lactic acid as the main metabolite. The maximum yields of 2.3 and 1.6 mol H /mol glucose were achieved for the 65 °C pretreated anaerobically digested and activated sludges, respectively. Approximately a 15% decrease in yield was observed with increasing pretreatment temperature from 65 to 95 °C concomitant with an increase in butyrate/acetate ratio from 1.5 to 2.4 for anaerobically digested sludge. The increase of pretreatment temperature of activated sludge to 95 °C suppressed the hydrogen production by lactic acid fermentation. DNA analysis of the microbial community showed that the elevated pretreatment temperatures reduced the species diversity. © 2008 International Association for Hydrogen Energy. 2