Electronic Thesis and Dissertation Repository


Master of Engineering Science


Chemical and Biochemical Engineering


Rehmann, Lars


Haematococcus pluvialis (H. pluvialis) is one of the main sources of astaxanthin, a high value carotenoid and a strong antioxidant. Astaxanthin extraction is hindered by the thick cell wall of H. pluvialis which is composed of sporopollenin. Mechanical cell disruption methods, high cost and energy-consuming, are required to enhance astaxanthin extraction. This study employed a novel technology using deep eutectic solvents (DESs) to demonstrate the possibility of direct extraction of astaxanthin without a disruption step. It was established that 68% of astaxanthin can be extracted from intact H. pluvialis cells using choline chloride-glycerol (ChCl-G) mixture with 12.5% (v/v ) water at 70 °C. Using deep eutectic solvents is a promising method for efficient extraction of astaxanthin from H. pluvialis, while complying with Green Chemistry Principles.