Electronic Thesis and Dissertation Repository


Master of Science


Physiology and Pharmacology


Dr. Lina Dagnino


Integrin-linked kinase (ILK) is a ubiquitous scaffold protein essential for the development of front-rear polarity and directional migration of epidermal keratinocytes. β-arrestin 1 is another adaptor protein which has recently emerged as a key factor in modulating proliferation and migration of various cell types. Previous studies have demonstrated an association between β-arrestin 2 and ILK in cerebellar granule precursor cells. I have now identified a novel interaction between ILK and β-arrestin 1 in primary keratinocytes that occurs directly and without post-translational modifications. The N-terminal 67 residues of ILK and multiple regions in β-arrestin 1 are important for this interaction. This association occurs regardless of the conformational state of β-arrestin 1. Furthermore, ILK and β-arrestin 1 colocalize at cell extensions, suggesting a possible role for this complex in the development of front-rear cell polarity and migration. My analysis of this novel interaction provides new insight into mechanisms of keratinocyte regulation.