Development of a Gallium-Containing One-Bead One-Compound Peptide Library for the Discovery of New Molecular Imaging Probes

Author

Fernanda C. Bononi, The University of Western OntarioFollow

Degree

Master of Science

Program

Chemistry

Supervisor

Leonard G Luyt

Abstract

An eight amino acid one-bead one-compound (OBOC) peptide library, containing a gallium-DOTA complex, was developed in an attempt to overcome common issues associated with the later addition of radionuclides to peptide chains of imaging probes. The further addition of a radionuclide often changes the binding properties of a peptide, as it adds bulk, and possibly charges. MALDI (matrix-assisted laser desorption ionization) tandem mass spectrometry was determined to be the method of choice in order to deconvolute gallium-containing peptide sequences. The library obtained was screened against the breast cancer cell lines MDA-MB-231 and MCF-7. Positive beads were isolated and sequences were determined before ligand validation, which included synthesis of peptides and further testing for binding affinity and specificity. Furthermore, positive peptides obtained through screening were developed into potential imaging agents by radiolabelling with ⁶⁸Ga.

Recommended Citation

Bononi, Fernanda C., "Development of a Gallium-Containing One-Bead One-Compound Peptide Library for the Discovery of New Molecular Imaging Probes" (2014). Electronic Thesis and Dissertation Repository. 2194.
https://ir.lib.uwo.ca/etd/2194