Author

Mirjam Urb

Date of Award

2006

Degree Type

Thesis

Degree Name

Master of Science

Program

Biology

Supervisor

Dr. R. Greg Thom

Abstract

Caterpillars, the larvae of Lepidoptera, are the main herbivores in many forests of ? ; . ! j i i 1 ! 4 : : the world. Foliage-feeding caterpillars also consume microbes, including fungi, occurring on and in leaves. If these fungi pass unharmed through the digestive tract, it should be possible to culture them also from caterpillar guts and frass. The purpose of this study was to assess and compare fungal communities associated with two Saturniidae caterpillars foraging on six host plants in the dry forest of Area de Conservaciôn Guanacaste, Costa Rica. Morphological and molecular identification of 835 cultures isolated revealed 48 genera and 123 species. Aspergillus, Cladosporium, Pestalotiopsis, Pénicillium, Fusarium, Phoma and Trichoderma were the most frequently isolated genera, but many species and genera were detected only once or twice. Although fungal communities from all four sources were similar in composition, the gut of Rothschildialebeau yielded the greatest diversity and estimated species richness, and frass yielded the least

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