Date of Award


Degree Type


Degree Name

Master of Engineering Science


Chemical and Biochemical Engineering


Dr. Argyrios Margaritis


The production of the biopolymer polyglutamic acid, using Bacillus licheniformis within a continuous stirred tank bioreactor was studied. This study into the continuous production of polyglutamic acid is the first to be reported. The effect of varying the dilution rate on the biomass concentration, the polyglutamic acid concentration and the substrates concentration were measured and the data were analyzed and correlated. The biomass productivity peaked at 0.16 g/L/h, and the poly glutamic acid productivity reached 1.2 g/L/h. The poly glutamic acid yield from the total carbon sources reached 0.33 g/g. The specific growth as a function of the total substrate concentration was fitted using the Richard - Margaritis empirical model. The maximum specific growth rate was 0.13 h-11 the saturation constant was 59.1 g/L and the relative coefficient was 4.3.



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