Chemistry Publications

Document Type

Article

Publication Date

11-8-2022

Journal

Analytical chemistry

Volume

94

Issue

44

First Page

15499

Last Page

15509

URL with Digital Object Identifier

10.1021/acs.analchem.2c03931

Abstract

Differential scanning calorimetry (DSC) is a standard tool for probing the resilience of monoclonal antibodies (mAbs) and other protein therapeutics against thermal degradation. Unfortunately, DSC usually only provides insights into global unfolding, although sequential steps are sometimes discernible for multidomain proteins. Temperature-dependent hydrogen/deuterium exchange (HDX) mass spectrometry (MS) has the potential to probe heat-induced events at a much greater level of detail. We recently proposed a strategy to deconvolute temperature-dependent HDX data into contributions from local dynamics, global unfolding/refolding, as well as chemical labeling. However, that strategy was validated only for a small protein (Tajoddin, N. N.; Konermann, L.

Available for download on Sunday, December 31, 2023

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