CelluSpots: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity

Authors

Chenggang Wu, University of Western Ontario
Shawn S.-C. Li, University of Western Ontario

Document Type

Book Chapter

Publication Date

8-1-2009

Journal

Methods in Molecular Biology Series

Volume

570

First Page

197

Last Page

202

URL with Digital Object Identifier

http://dx.doi.org/10.1007/978-1-60327-394-7_8

Abstract

Peptide arrays differ from conventional peptide synthesis in that hundreds upon thousands of peptides are synthesized and presented on a planar surface at a time. While direct synthesis of peptide arrays on a functionalized surface is feasible, reprinting of pre-made peptides offers flexibility and reproducibility and drastically reduces cost when multiple copies of the same or related peptide arrays are needed. CelluSpot, a method developed by Intavis, opens a new route in peptide array synthesis and printing and overcomes certain limitations of the SPOT membrane. This technique was used to produce hundreds of phosphotyrosine-oriented peptide array libraries for determining the specificity of the Src homology 2 (SH2) domain.

Notes

Published as a book chapter in: Peptide Microarrays: Methods and Protocols (Vol. 570 of the Methods in Molecular Biology Series).