Automatic detection of pale path and overlaps in chromosome images using adaptive search technique and re-thresholding
VISAPP 2012 - Proceedings of the International Conference on Computer Vision Theory and Applications
Detection and separation of overlapping and touching chromosomes is a critical issue in image analysis applications for cytogenetics where accurately segmented chromosomes are essential. We present a novel method of automatic pale path detection for all types of stained chromosome images. Optimum number of cut-points for each cluster of touching or overlapping chromosomes is obtained and analysed sequentially for the pale paths. A self-adaptive search window searches for the minimum grayscale intensity beginning from the vicinity of a cut-point and propagates gradually till the end of pale path. Efficient image and area thresholding restricts the faulty detection of touch or overlap in a chromosome cluster.