Anatomy and Cell Biology Publications

Title

Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures

Authors

Caasy Porch-Thomas, Biomedical Science Program, Tulane University School of Medicine & Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
Jie Li, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine & National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University
Fabiana Zanata, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine & Federal University of Sao Paulo
Elizabeth C. Martin, Department of Biological and Agricultural Engineering, Louisiana State University
Nicholas Pashos, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
Kaylynn Genemaras, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
Nicholas J. Poche, Department of Biological and Agricultural Engineering, Louisiana State University
Nicholas P. Totaro, Department of Biological and Agricultural Engineering, Louisiana State University
Melyssa R. Bratton, Department of Chemistry, Xavier University of Louisiana
Dina Gaupp, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
Trivia Frazier, Center for Stem Cell Research & Regenerative Medicine, Department of Structural and Cell Biology, , Tulane University School of Medicine & LaCell LLC, New Orleans, Louisiana
Xiying Wu, LaCell LLC, New Orleans, Louisiana
Lydia Masako Ferreira, Federal University of Sao Paulo
Weidong Tian, National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University
Guangdi Wang, Department of Chemistry, Xavier University of Louisiana
Bruce A. Bunnell, Center for Stem Cell Research & Regenerative Medicine, Department of Pharmacology, Tulane University School of Medicine
Lauren Flynn, Department of Chemical and Biochemical Engineering, Department of Anatomy and Cell Biology, Western University
Daniel Hayes, Department of Biomedical Engineering, Pennsylvania State University
Jeffrey M. Gimble, Department of Surgery, Tulane University School of Medicine

Document Type

Article

Publication Date

9-2018

Issue

9

Journal

Journal of Biomedical Materials Research Part A

Volume

106

First Page

2481

Last Page

2493

URL with Digital Object Identifier

https://doi.org/10.1002/jbm.a.36444

Abstract

Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft tissue cosmesis, grafting, and reconstruction. Adipose tissue decellularization has been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct decellularization processes may yield scaffolds with differing compositions, the current study employed mass spectrometry to compare the proteomes of human adipose-derived matrices generated through three independent methods combining enzymatic-, detergent-, and/or solvent-based steps. In addition to protein content, bioscaffolds were evaluated for deoxyribose nucleic acid depletion, extracellular matrix composition, and physical structure using optical density, histochemical staining, and scanning electron microscopy. Mass spectrometry based proteomic analyses identified 25 proteins (having at least two peptide sequences detected) in the scaffolds generated with an enzymatic approach, 143 with the detergent approach, and 102 with the solvent approach, as compared to 155 detected in unprocessed native human fat. Immunohistochemical detection confirmed the presence of the structural proteins actin, collagen type VI, fibrillin, laminin, and vimentin. Subsequent in vivo analysis of the predominantly enzymatic- and detergent-based decellularized scaffolds following subcutaneous implantation in GFP(+) transgenic mice demonstrated that the matrices generated with both approaches supported the ingrowth of host-derived adipocyte progenitors and vasculature in a time dependent manner. Together, these results determine that decellularization methods influence the protein composition of adipose tissue-derived bioscaffolds. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2481-2493, 2018.

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