Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Surgery

Supervisor

Pautler, Dr. Stephen

2nd Supervisor

Power, Dr. Nicholas

Co-Supervisor

3rd Supervisor

Leong, Dr. Hon

Co-Supervisor

Abstract

Prostate cancer remains a substantial contributor to cancer-related mortality worldwide. Current screening methods include obtaining a PSA blood test. However, controversy surrounds its use as it is neither sensitive nor specific.

Nanoscale flow cytometry is a type of microfluidics-based technology that allows enumeration of submicron tumor fragments known as microparticles (MPs). In this study, prostate specific microparticles in patient plasma were targeted using fluorophore-conjugated antibodies. Targeted cell surface antigens or biomarkers include: prostate specific membrane antigen (PSMA), six-transmembrane epithelial antigen of the prostate-1 (STEAP1), ghrelin receptor (GHSR1a) and CD151.

A statistically significant difference in the level of MP levels was measured with PSMA+STEAP1+GHSR1a and PSMA+STEAP1+CD151 triple-expressing MPs when comparing Gleason score (GS) 6 to GS3+4, GS4+3 and GS≥8 cohorts. In this pilot and exploratory study, I show that MPs have the potential of becoming a “liquid biopsy” that can assist in risk stratification prior to a prostate needle biopsy.


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