Electronic Thesis and Dissertation Repository

Degree

Master of Engineering Science

Program

Biomedical Engineering

Supervisor

Dr. Douglas Hamilton

Abstract

Clinical therapies for the treatment of periodontitis are unable to reproducibly stimulate regeneration of the periodontium. We assessed the use of a novel electrospun type I collagen scaffold containing recombinant periostin to stimulate regeneration of the periodontal ligament (PDL) and bone. Human PDL cells demonstrated the ability to form a mineralized matrix in vitro and reduced osteogenic potential with increased donor age. In vitro analysis indicated scaffolds were biocompatible, however, periostin did not significantly influence adhesion or growth. In the healing of fenestration defects in rats, type I collagen scaffolds (with and without periostin) initially delayed cell infiltration and increased M2 macrophage recruitment, indicating either an elevated healing response or foreign body reaction. The incorporation of periostin into scaffolds had a limited effect on inflammation, collagen synthesis, and bone formation in vivo. Further optimization of periostin concentration and/or scaffold design is required to determine whether recombinant periostin influences periodontal healing.


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