Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Kinesiology

Supervisor

Dr.Earl Noble

Abstract

The purpose of this study was to determine the effect of exercise training alone and in a model of Type 1 Diabetes Mellitus (T1DM), on Heat Shock Protein (HSP) and Nitric Oxide Synthase (NOS) expression in entorhinal adjacent large and small cerebral vessels. Thirty-two rats were randomly allocated to four groups: control sedentary (C), control exercised (CX), diabetic sedentary (D) and diabetic exercised (DX). Exercise training incorporated 5 days/week on a motorized treadmill (27m/min; 6 degree incline; 1 hour) for 10 weeks. Exercise trained groups had significantly greater Hsp70 expression than their respective non-trained groups (p<0.05) and this response was not blunted in T1DM animals. The inducible NOS (iNOS) expression was greater in diabetic sedentary when compared to all other groups (p<0.001). Co-localization of protein with smooth muscle cells illustrates that all HSP and NOS signal content is localized to the smooth muscle area (SMA).


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