Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Microbiology and Immunology

Supervisor

Dr. Joe Mymryk

Abstract

The human adenovirus (HAdV) E1A protein is the first protein produced post-HAdV infection, and serves two main functions. The first is to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of interacting with the poorly characterized human DNA replication-related element-binding factor (hDREF). hDREF is a transcription factor associated with the expression of several genes related to the cell cycle. I hypothesized that the interaction between E1A and hDREF would contribute to adenovirus induced transcriptional modulation and viral replication in HAdV-5 infected host cells.

Utilizing co-immunoprecipitation experiments, I discovered that E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or not the E1A-hDREF interaction is specifically responsible for these observed results.


Included in

Virology Commons

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