Location

London

Event Website

http://www.csce2016.ca/

Description

The study discussed here is an attempt at comparing three of the most important kinetic parameters in an enzyme catalysed aerobic system; overall reaction kinetic constant, hydrolysis rate constant and oxygen assimilation constant. The kinetic parameters are compared with each other as well as between uncatalyzed and enzyme catalyzed reactions. Theatrical reaction kinetic models were developed for the analysis. Batch experiments were conducted to characterize solid waste behaviour and the results used to calibrate the developed models. Lignin content, cellulose/hemicellulose content, total organic content, oxygen depletion and CO2 production was used as responses in the experiments. Manganese peroxidase from white-rot-fungi is used as the enzyme for catalysing treatments. The catalyzed reactions showed higher reaction rates than the un-catalyzed reactions. Further analysis revealed that catalyzed reactions have higher hydrolysis rates compared to the overall rates of metabolism. The O2 assimilation analysis revealed that catalyzed reactions require 1.66 times more O2 than un-catalyzed reactions.


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Jun 1st, 12:00 AM Jun 4th, 12:00 AM

ENV-623: ANALYSIS OF REACTION KINETIC PARAMETERS IN ENZYME CATALYSED AEROBIC WASTE DEGRADATION

London

The study discussed here is an attempt at comparing three of the most important kinetic parameters in an enzyme catalysed aerobic system; overall reaction kinetic constant, hydrolysis rate constant and oxygen assimilation constant. The kinetic parameters are compared with each other as well as between uncatalyzed and enzyme catalyzed reactions. Theatrical reaction kinetic models were developed for the analysis. Batch experiments were conducted to characterize solid waste behaviour and the results used to calibrate the developed models. Lignin content, cellulose/hemicellulose content, total organic content, oxygen depletion and CO2 production was used as responses in the experiments. Manganese peroxidase from white-rot-fungi is used as the enzyme for catalysing treatments. The catalyzed reactions showed higher reaction rates than the un-catalyzed reactions. Further analysis revealed that catalyzed reactions have higher hydrolysis rates compared to the overall rates of metabolism. The O2 assimilation analysis revealed that catalyzed reactions require 1.66 times more O2 than un-catalyzed reactions.

http://ir.lib.uwo.ca/csce2016/London/Environmental/12