Chemistry Publications

Title

Cellular Localization and Allele-selective Inhibition of Mutant Huntingtin Protein by Peptide Nucleic Acid Oligomers Containing the Fluorescent Nucleobase [bis-o-(aminoethoxy)phenyl]pyrrolocytosine

Document Type

Article

Publication Date

11-2009

Journal

Bioorganic & Medicinal Chemistry Letters

Volume

19

Issue

21

First Page

6181

Last Page

6184

URL with Digital Object Identifier

10.1016/j.bmcl.2009.09.004

Abstract

Peptide nucleic acid (PNA) is a successful DNA/RNA mimic. A major challenge for research is to invent chemically modified PNAs that retain the favorable properties of the parent compound while improving biological recognition. Here, we test modified PNAs containing [bis-o-(aminoethoxy)phenyl]pyrrolocytosine bases designed to engage guanine with an additional hydrogen bond. We observe elevated melting temperatures, localization to cellular compartments, and allele-selective inhibition of mutant huntingtin protein expression.