An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene

Authors

Ewan A. Gibb, The University of Western Ontario
David R. Edgell, The University of Western Ontario

Document Type

Article

Publication Date

4-2009

Journal

Journal of Bacteriology

Volume

191

Issue

7

First Page

2409

Last Page

2413

URL with Digital Object Identifier

http://dx.doi.org/10.1128/JB.01751-08

Abstract

Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced beta-galactosidase activity, consistent with translational regulation.